About: HLA-A∗0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins

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About: HLA-A∗0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	HLA-A∗0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins
Creator	Jan, Jia-Tsrong Chu, Chen-Chung Chen, Show-Li Leng, Chih-Hsiang Lin, Jian-Yu »more»
source	Elsevier; Medline; PMC
abstract	Abstract The immunogenicity of HLA-A∗0201-restricted cytotoxic T lymphocyte (CTL) peptide in severe acute respiratory syndrome coronavirus (SARS-CoV) nuclear capsid (N) and spike (S) proteins was determined by testing the proteins’ ability to elicit a specific cellular immune response after immunization of HLA-A2.1 transgenic mice and in vitro vaccination of HLA-A2.1 positive human peripheral blood mononuclearcytes (PBMCs). First, we screened SARS N and S amino acid sequences for allele-specific motif matching those in human HLA-A2.1 MHC-I molecules. From HLA peptide binding predictions (http://thr.cit.nih.gov/molbio/hla_bind/), ten each potential N- and S-specific HLA-A2.1-binding peptides were synthesized. The high affinity HLA-A2.1 peptides were validated by T2-cell stabilization assays, with immunogenicity assays revealing peptides N223–231, N227–235, and N317–325 to be the first identified HLA-A∗0201-restricted CTL epitopes of SARS-CoV N protein. In addition, previous reports identified three HLA-A∗0201-restricted CTL epitopes of S protein (S978–986, S1203–1211, and S1167–1175), here we found two novel peptides S787–795 and S1042–1050 as S-specific CTL epitopes. Moreover, our identified N317–325 and S1042–1050 CTL epitopes could induce recall responses when IFN-γ stimulation of blood CD8+ T-cells revealed significant difference between normal healthy donors and SARS-recovered patients after those PBMCs were in vitro vaccinated with their cognate antigen. Our results would provide a new insight into the development of therapeutic vaccine in SARS.
has issue date	2006-05-26(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2006.03.152
bibo:pmid	16630549
has license	els-covid
sha1sum (hex)	20d91da4be960dad3519d5438db7295d97b80bcc
schema:url	https://doi.org/10.1016/j.bbrc.2006.03.152
resource representing a document's title	HLA-A∗0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins
has PubMed Central identifier	PMC7092919
has PubMed identifier	16630549
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:20d91da4be960dad3519d5438db7295d97b80bcc#body_text
is schema:about of	named entity 'PREVIOUS REPORTS' named entity 'PROTEIN ' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'IMMUNOGENICITY' named entity 'REVEALED' »more»

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