About: Production of IFN-γ in feline whole blood after incubation with potential T-cell epitopes of the nucleocapsid protein of feline coronavirus

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About: Production of IFN-γ in feline whole blood after incubation with potential T-cell epitopes of the nucleocapsid protein of feline coronavirus Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Production of IFN-γ in feline whole blood after incubation with potential T-cell epitopes of the nucleocapsid protein of feline coronavirus
Creator	Paltrinieri, S Battilani, M Cornaro, C Pocacqua, V Rossi, G
source	Elsevier; Medline; PMC
abstract	Interferon gamma (IFN-γ) plays an important role in cell mediated responses against mutated feline coronavirus strains (FCoV) involved in the pathogenesis of feline infectious peritonitis (FIP). The aim of this study was to establish a combined in silico and in vitro approach to assess feline leukocyte production of IFN-γ in response to selected peptides of the nucleocapside protein (N) of FCoVs. To this aim, we designed, through a bioinformatic approach, 8 potentially immunogenic peptides from the protein N corresponding to sequences of residues 14, 182, 198 detected only in FCoVs from FIP cats (virulent strains), only in FCoVs from healthy cats (avirulent strains) and both in FIP and in healthy cats (mixed strains). The peptides or a sham solution were incubated with whole blood from 16 cats (7 healthy and 9 with chronic diseases other than FIP) and IFN-γ concentration was measured on plasma using an ELISA system. RT-PCR expression of IFN-γ mRNA was also evaluated after incubation of the peptides or a sham solution with whole blood from 4 clinically healthy cats. The mean plasma concentration of IFN-γ in samples incubated with peptides decreased and the expression of IFN-γmRNA did not change compared with the sham solution, except for some cats with chronic diseases (which probably have a “pre-activated” immune response). These cats responded to “avirulent” or “mixed” peptides by increasing the concentration of IFN-γ and the expression of IFN-γ mRNA. The combined approach employed in this study allowed us to identify potentially immunogenic peptides of FCoV N protein that can modulate the production of IFN-γ especially in cats with a “pre-activated” cell mediated response.
has issue date	2011-06-02(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2011.02.004
bibo:pmid	21376481
has license	no-cc
sha1sum (hex)	20a2e0c4bf75fe9109100948896ebcdbbdd36e82
schema:url	https://doi.org/10.1016/j.vetmic.2011.02.004
resource representing a document's title	Production of IFN-γ in feline whole blood after incubation with potential T-cell epitopes of the nucleocapsid protein of feline coronavirus
has PubMed Central identifier	PMC7117359
has PubMed identifier	21376481
schema:publication	Vet Microbiol
resource representing a document's body	covid:20a2e0c4bf75fe9109100948896ebcdbbdd36e82#body_text
is schema:about of	named entity 'IFN-g' named entity 'feline' named entity 'T-cell' named entity 'blood' named entity 'feline coronavirus' »more»

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