About: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review
Creator	Sozzi, Emanuele Wuertz, Stefan Sirikanchana, Kwanrawee Dan, Cheng Shoults, David
source	MedRxiv
abstract	Capsid integrity quantitative PCR (qPCR), a molecular detection method for infectious viruses combining azo dye pretreatment with qPCR, has been widely used in recent years; however, variations in pretreatment conditions for various virus types can limit the efficacy of specific protocols. By identifying and critically synthesizing 42 recent peer-reviewed studies employing capsid integrity qPCR for viruses in the last decade (2009 to 2019) in the fields of food safety and environmental virology, we aimed to establish recommendations for the detection of infectious viruses. Intercalating dyes are effective measures of viability in PCR assays provided the viral capsid is damaged; viruses that have been inactivated by other causes, such as loss of attachment or genomic damage, are less well detected using this approach. Although optimizing specific protocols for each virus is recommended, we identify a framework for general assay conditions. These include concentrations of ethidium monoazide, propidium monoazide or its derivates between 10 and 200 uM; incubation on ice or at room temperature (20 to 25C) for 5 to 120 min; and dye activation using LED or high light (500 to 800 Watts) exposure for periods ranging from 5 to 20 min. These simple steps can benefit the investigation of infectious virus transmission in routine (water) monitoring settings and during viral outbreaks such as the current COVID-19 pandemic or endemic diseases like dengue fever.
has issue date	2020-05-12(xsd:dateTime)
bibo:doi	10.1101/2020.05.08.20095364
has license	medrxiv
sha1sum (hex)	1ebb1ac87f5ed3190e38b188f1ce5f8560789c06
schema:url	https://doi.org/10.1101/2020.05.08.20095364
resource representing a document's title	Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications; a systematic review
resource representing a document's body	covid:1ebb1ac87f5ed3190e38b188f1ce5f8560789c06#body_text
is schema:about of	named entity 'viruses' named entity 'dyes' named entity 'specific' named entity 'assays' named entity 'These' »more»

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software