About: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

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About: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
Creator	Kitajima, Masaaki Haramoto, Eiji Malla, Bikash Thakali, Ocean
source	Elsevier; Medline; PMC
abstract	Abstract Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200–5000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 103 copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 × 103–8.2 × 104 copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 × 102–2.5 × 103 copies/L) with a higher filtration volume of 5000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.
has issue date	2020-06-20(xsd:dateTime)
bibo:doi	10.1016/j.scitotenv.2020.140405
bibo:pmid	32783878
has license	els-covid
sha1sum (hex)	1bcdcbfa44979c90c2e8f3ab7d26e7e77446ce01
schema:url	https://doi.org/10.1016/j.scitotenv.2020.140405
resource representing a document's title	First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
has PubMed Central identifier	PMC7305903
has PubMed identifier	32783878
schema:publication	Sci Total Environ
resource representing a document's body	covid:1bcdcbfa44979c90c2e8f3ab7d26e7e77446ce01#body_text
is schema:about of	named entity 'presence' named entity 'presence' named entity 'wastewater' named entity 'RNA' named entity 'PROOF' »more»

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