About: Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates

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About: Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates
Creator	Kim, Tong-Soo Shin, Ho-Joon Kang, Jung-Mi Lee, Jinyoung Moe, Mya »more»
source	PMC
abstract	BACKGROUND: Plasmodium falciparum merozoite surface protein-3 (PfMSP-3) is a target of naturally acquired immunity against P. falciparum infection and is a promising vaccine candidate because of its critical role in the erythrocyte invasion of the parasite. Understanding the genetic diversity of pfmsp-3 is important for recognizing genetic nature and evolutionary aspect of the gene in the natural P. falciparum population and for designing an effective vaccine based on the antigen. METHODS: Blood samples collected from P. falciparum-infected patients in Naung Cho and Pyin Oo Lwin, Myanmar, in 2015 were used in this study. The pfmsp-3 was amplified by polymerase chain reaction, cloned, and sequenced. Genetic polymorphism and natural selection of Myanmar pfmsp-3 were analysed using the programs DNASTAR, MEGA6, and DnaSP 5.10.00. Genetic diversity and natural selection of the global pfmsp-3 were also comparatively analysed. RESULTS: Myanmar pfmsp-3 displayed 2 different alleles, 3D7 and K1. The 3D7 allelic type was predominant in the population, but genetic polymorphism was less diverse than for the K1 allelic type. Polymorphic characters in both allelic types were caused by amino acid substitutions, insertions, and deletions. Amino acid substitutions were mainly occurred at the alanine heptad repeat domains, whereas most insertions and deletions were found at the glutamate rich domain. Overall patterns of amino acid polymorphisms detected in Myanmar pfmsp-3 were similar in the global pfmsp-3 population, but novel amino acid changes were observed in Myanmar pfmsp-3 with low frequencies. Complicated patterns of natural selection and recombination events were predicted in the global pfmsp-3, which may act as major driving forces to maintain and generate genetic diversity of the global pfmsp-3 population. CONCLUSION: Global pfmsp-3 revealed genetic polymorphisms, suggesting that the functional and structural consequences of the polymorphisms should be considered in designing a vaccine based on PfMSP-3. Further examination of genetic diversity of pfmsp-3 in the global P. falciparum population is necessary to gain in-depth insight for the population structure and evolutionary aspect of global pfmsp-3.
has issue date	2020-05-19(xsd:dateTime)
bibo:doi	10.1186/s12936-020-03256-y
bibo:pmid	32429986
has license	cc-by
sha1sum (hex)	1b308b3d8793e83c013d5bd7291c3d4cc367e5e3
schema:url	https://doi.org/10.1186/s12936-020-03256-y
resource representing a document's title	Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates
has PubMed Central identifier	PMC7235555
has PubMed identifier	32429986
schema:publication	Malar J
resource representing a document's body	covid:1b308b3d8793e83c013d5bd7291c3d4cc367e5e3#body_text
is schema:about of	named entity 'CRITICAL' covid:arg/1b308b3d8793e83c013d5bd7291c3d4cc367e5e3 named entity 'vaccine' named entity 'infection' named entity 'critical role' »more»

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