About: Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

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About: Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
Creator	Lin, Chii-Wann Chang, Chia-Chen Ee, Tan Lin, Wei-Yin Wu, Tzu-Heng »more»
source	Medline; PMC
abstract	In this work, hybridization chain reactions (HCRs) toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS–CoV-2) nucleocapsid phosphoproteins gene loci and human RNase P are proposed to provide an isothermal amplification screening tool. The proposed chain reactions target the complementary DNA (cDNA) of SARS–CoV-2, with loci corresponding to gold-standard polymerase chain reaction (PCR) loci. Four hybridization chain reaction reactions are demonstrated herein, targeting N1/N2/N3 loci and human RNase P. The design of the hybridization chain reaction, herein, is assisted with an algorithm. The algorithm helps to search target sequences with low local secondary structure and high hybridization efficiency. The loop domain of the fuel hairpin molecule H1 and H2, which are the tunable segments in such reactions, are used as an optimization parameter to improve the hybridization efficiency of the chain reaction. The algorithm-derived HCR reactions were validated with gel electrophoresis. All proposed reactions exhibit a hybridization complex with a molecular mass >1.5k base pairs, which is clear evidence of chain reaction. The hybridization efficiency trend revealed by gel electrophoresis corresponds nicely to the simulated data from the algorithm. The HCR reactions and the corresponding algorithm serve as a basis to further SARS–CoV-2 sensing applications and facilitate better screening strategies for the prevention of on-going pandemics.
has issue date	2020-05-01(xsd:dateTime)
bibo:doi	10.3390/ijms21093216
bibo:pmid	32370065
has license	cc-by
sha1sum (hex)	13db3844bdeb2d3acc5fe5a7b94e8ebff09eb1e5
schema:url	https://doi.org/10.3390/ijms21093216
resource representing a document's title	Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
has PubMed Central identifier	PMC7246904
has PubMed identifier	32370065
schema:publication	Int J Mol Sci
resource representing a document's body	covid:13db3844bdeb2d3acc5fe5a7b94e8ebff09eb1e5#body_text
is schema:about of	named entity 'polymerase' named entity 'efficiency' named entity 'amplification' named entity 'complementary DNA' named entity 'serve' »more»

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