About: Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice

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About: Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice
Creator	Gao, Yang Zhao, Wei Yu, Hua Fang, Dan-Yun Jiang, Li-Fang »more»
source	Elsevier; Medline; PMC
abstract	Abstract Antibodies to SARS-Coronavirus (SARS-CoV)-specific B cell epitopes might recognize the pathogen and interrupt its adherence to and penetration of host cells. Hence, these epitopes could be useful for diagnosis and as vaccine constituents. Using the phage-displayed peptide library screening method and purified Fab fragments of immunoglobulin G (IgG Fab) from normal human sera and convalescent sera from SARS-CoV-infected patients as targets, 11 B cell epitopes of SARS-CoV spike glycoprotein (S protein) and membrane protein (M protein) were screened. After a bioinformatics tool was used to analyze these epitopes, four epitope-based S protein dodecapeptides corresponding to the predominant epitopes were chosen for synthesis. Their antigenic specificities and immunogenicities were studied in vitro and in vivo. Flow cytometry and ELISPOT analysis of lymphocytes as well as a serologic analysis of antibody showed that these peptides could trigger a rapid, highly effective, and relatively safe immune response in BALB/c mice. These findings might aid development of SARS diagnostics and vaccines. Moreover, the role of S and M proteins as important surface antigens is confirmed.
has issue date	2007-03-15(xsd:dateTime)
bibo:doi	10.1016/j.virol.2006.09.016
bibo:pmid	17055022
has license	els-covid
sha1sum (hex)	0fe4c61765775f05ecce8cc192fb3cfff30c8dff
schema:url	https://doi.org/10.1016/j.virol.2006.09.016
resource representing a document's title	Selection of SARS-Coronavirus-specific B cell epitopes by phage peptide library screening and evaluation of the immunological effect of epitope-based peptides on mice
has PubMed Central identifier	PMC7103350
has PubMed identifier	17055022
schema:publication	Virology
resource representing a document's body	covid:0fe4c61765775f05ecce8cc192fb3cfff30c8dff#body_text
is schema:about of	named entity 'COULD BE' named entity 'M PROTEINS' named entity 'PENETRATION' named entity 'TRIGGER' named entity 'SARS' »more»

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