About: Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS

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About: Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS
Creator	Kawaoka, Yoshihiro Wang, I-Hsuan Ueki, Hiroshi Gunzer, Matthias Zhao, Dongming
source	Medline; PMC
abstract	In vivo two-photon imaging is a valuable technique for studies of viral pathogenesis and host responses to infection in vivo. In this protocol, we describe a methodology for analyzing influenza virus–infected lung in vivo by two-photon imaging microscopy. We describe the surgical procedure, how to stabilize the lung, and an approach to analyzing the data. Further, we provide a database of fluorescent dyes, antibodies, and reporter mouse lines that can be used in combination with a reporter influenza virus (Color-flu) for multicolor analysis. Setup of this model typically takes ~30 min and enables the observation of influenza virus–infected lungs for >4 h during the acute phase of the inflammation and at least 1 h in the lethal phase. This imaging system, which we termed two-photon IMPRESS (imaging pathophysiology research system), is broadly applicable to analyses of other respiratory pathogens and reveals disease progression at the cellular level in vivo.
has issue date	2020-01-29(xsd:dateTime)
bibo:doi	10.1038/s41596-019-0275-y
bibo:pmid	31996843
has license	no-cc
sha1sum (hex)	05e325aff18c0a3d1b808e794e11c130bbf064d1
schema:url	https://doi.org/10.1038/s41596-019-0275-y
resource representing a document's title	Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS
has PubMed Central identifier	PMC7086515
has PubMed identifier	31996843
schema:publication	Nat Protoc
resource representing a document's body	covid:05e325aff18c0a3d1b808e794e11c130bbf064d1#body_text
is schema:about of	named entity 'respiratory' named entity 'influenza' named entity 'influenza' named entity 'virus' named entity 'vivo' »more»

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